Thermo Fisher Scientific inc., the world leader in serving science, presented two posters at this year’s Association for Laboratory Automation conference and exhibition (ALA):

Multiskan FC: High-Throughput Enzyme Linked Immunosorbant Assay Performed by Molecule Immobilization on the Surface of Magnetic Particles

This paper describes the use of the Thermo Scientific Multiskan FC in the detection of atrazine using enzyme linked immunosorbent assay (ELISA) by molecule immobilization on the surface of magnetic particles, and the separation of the particle bound complexes with the Thermo Scientific KingFisher Flex instrument, a magnetic particle processor. The ELISAs based on magnetic particle separation technique are faster to perform than the traditional ELISAs, which require more time-consuming separation steps of the unbound molecules.

Varioskan Flash: Internal Calibration of Microplate Reader Detection System to Improve the Sensitivity, Dynamic Range and the Accuracy of the Spectral Scanning in Fluorometry and Luminometry

When fluorescence signals are measured the results are normally referred as “Relative Fluorescence Units” (RFUs) to emphasis the fact that resulting numeric values are heavily dependent on the settings of the instrument electronics, mainly PMT gain voltage. Results are also somewhat dependent on the environmental aspects, like ambient temperature that affects the electronic noise levels. The practical effect of this relative nature of fluorescence measurements is that result values become quite difficult to be compared, especially if the results are from different measurements done with different instrument settings. Thermo Scientific Varioskan Flash® multi-technology reader has in-built calibration system for all measurement technologies covering all mentioned problems in the measurement. This paper shows how detection systems for fluorescence intensity, time resolved fluorescence and luminescence are calibrated to ensure the best possible performance in each individual measurement.