Optimization of Instrument Settings and Measurement Parameters for Kinetic Fluorometric and Luminometric Assays

The speed of reaction kinetics of fluorometric and luminometric assays sets certain important requirements for the detection instrument and measurement paramaters.  Fluorometric peroxide / peroxidase assay, luminometric ATP assay and luminescence producing reastion between Aequorin and calcium were used as examples for optimizing measurements of differnt types of kinetic reactions in microplate format.  The results present many possible way of selecting optimal instrument settings according to the reaction speed.