Ultrasensitive Luminometric ATP Assay with Luminoskan Ascent® and Flash Type ATP Chemistry

Moles ATP / well is a very common way to determine luminometer sensitivity. However, there are several different assays and assay types for measuring ATP. Especially the assay type; flash or glow, moves the system to a very different sensitivity range. The theoretical sensitivity values for Thermo Scientific Luminoskan Ascent and Fluoroskan Ascent FL instruments were originally given based on a glow ATP assay. Recently, the performance test procedure was changed to utilize flash ATP assay, therefore the new and old sensitivity values differ remarkably. The purpose of this note is to describe the difference and reason between the old and new luminometric sensitivity specifications of Thermo Scientific Luminoskan Ascent and Fluoroskan Ascent FL luminometers.