TRF measurements with Varioskan®

Varioskan is a spectral scanning multimode reader capable of photometric, fluorometric and time-resolved fluorometric measurements. This sheet will give a short description how to perform different time-resolved fluorometric (TRF) assays with Varioskan.

Time-resolved fluorescence means that the measurement of the specific fluorescence emission signal is started after a delay. During the delay the short-lived background fluorescence has decayed. The following table contains a short description of each TRF measurement type. The parameters given on the table are only the default values for Europium and Europium-APC pair and need to be determined specifically for each assay.