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Comparison of Flash and Glow ATP Assays with Thermo Scientific Varioskan Flash Luminometry
Jorma Lampinen, Marika Raitio, Arto Perälä, Vuokko Kytöniemi and Reija-Riitta Harinen Thermo Fisher Scientific Oy, Vantaa, Finland
Abstract
One of the most common luminescent reactions used in research is an ATP reaction catalysed by firefly luciferases. In this reaction ATP is degraded to AMP and the energy is released as visible light. ATP reaction can be used for the measurement of ATP (1-3), its degradation products ADP and AMP (4) or any enzyme activity that can be linked to ATP production or consumption (5). It can also be used for the measurement of firefly enzyme activity in applications where firefly luciferase gene is used as a reporter gene (6-9).