Quantitative Determination of Total Bile Acids with a Thermo Scientific Varioskan Flash Multimode Reader Using a Kinetic Enzyme Cycling Assay

Aldo Roda, Department of Pharmaceutical Sciences, University of Bologna, Italy
Jorma Lampinen, Thermo Fisher Scientific, Vantaa, Finland

Serum total bile acids (TBA) level is a long-established and accepted indicator of liver diseases. This application note presents a simple photometric measurement of serum TBA.

TBA concentration was determined with an enzyme cycling assay kit (Total Bile Acid Assay kit, DZ042A, Diazyme Laboratories, Poway, CA). This kit is designed for cuvette photometer use but for this study the kit was adapted to the 96-well microtiter plate format. This reduces the consumption of the reagents and therefore the assay costs at least 50% and at the same time increases the throughput
remarkably since up to 43 samples can be analyzed simultaneously.