Quantitative Determination of Progesterone in Serum Samples with a Thermo Scientific Varioskan Flash Multimode Reader Using an Enzyme-Linked Immunosorbent Assay

Mara Mirasoli, Aldo Roda, Department of Pharmaceutical Sciences, University of Bologna, Italy
Jorma Lampinen, Thermo Fisher Scientific, Vantaa, Finland

Enzyme-linked immunosorbent assays (ELISA) are widely used in the clinical, agrofood and environmental fields. By taking advantage of the high binding avidity and selectivity of antigenantibody binding, these assays allow accurate and sensitive analyte quantification with little or no pre analytical sample treatment.