High Sensitivity ELISA Assays for the Detection of Melamine Residuals in Milk

This note describes how melamine screening from milk samples can be easily performed using simple ELISA assay with high sensitivity. The total assay time is about one hour and only standard  ELISA instrumentation is needed. The required instrumentation is Thermo Scientific Multiskan FC microplate photometer (or any ther Multiskan photometer) with Thermo Scientific Wellwash AC microplate washer (Wellwash 4 Mk 2 can also be used) and Thermo Scientific MultidropCombi microplate dispenser. In addition, the ELISA system can easily be fully automated using, as an example, the Thermo Scientific Catalyst Express robotics and supporting software. With this instrument combination it is possible to detect below 10 ìg/kg melamine residuals in milk samples.