Kinetic Measurements of Antibiotic Susceptibility Studies with a Microplate Photometer

Reija-Riitta Harinen, Jorma Lampinen, Marika Raitio and Vuokko Kytöniemi, Thermo Fisher Scientific, Microplate Instrumentation, Vantaa, Finland

In this paper we describe how a filter based microplate photometer, Thermo Scientific Multiskan FC, can be used reliably and easily to measure turbidity of bacterial samples in antibiotic susceptibility studies. The growth curve measurements were performed on a 96-well microplate. The performance of turbidometric measurements on a 96-well microplate and the liquid evaporation from 96- and 384-well microplates during long incubation were also studied.