Using a Thermo Scientific Microplate Photometer to Choose an Optimum Reagent for Cytotoxicity Assays

Abstract

A number of different reagents can be used to perform photometric cell proliferation and viability assays. A microplate photometer can quickly determine which reagent is the best choice for an assay, and then optimize the assay. Significant time savings and productivity gains can be achieved with this tool.

This paper explains how three different cell proliferation reagents were tested on MG87 murine fibroblast cells. The tested reagents were commonly used tetrazolium salts and alamarBlue® dye.

Reduction and metabolism rates were detected photometrically using the Thermo Scientific Multiskan FC microplate photometer.