Evaluation of Rapid ELISA Assay System for Detection of Melamine Residuals in Milk

This paper describes how melamine screening from milk samples can be easily performed using simple ELISA assay with high sensitivity. The performance of two commercial melamine ELISA assays was evaluated with basic ELISA system including a microplate washer, dispenser and a photometer from Thermo Fisher Scientific. This ELISA system can also be easily fully automated using for example Thermo Scientific Catalyst Express robotics and supporting software. With this instrument combination it is possible to detect below 10 ìg/kg melamine residuals from milk samples.