Luminometric Sensitivity of the Thermo Scientific Appliskan^® Multimode Reader

MIB Application Laboratory, Thermo Fisher Scientific, Vantaa, Finland

Introduction

Luminescence is the emission of light at visible wavelength by a substance. Excitation light is not required because the excitation energy is caused by a chemical or biochemical reaction. Luminescence reactions can be divided into two types according to the duration of the light emission. In flash reactions the light emission decays rapidly lasting only for a couple of seconds. Glow luminescence, on the other hand, can last for hours because light decays very slowly.