High sensitivity DNA and protein analysis with Thermo Scientific Multiskan Spectrum® and Thermo Scientific Microtiter® UV Microplates

Abstract

This paper shows how Multiskan Spectrum® microplate spectrophotometer together with Microtiter® UV Microplates can be used for DNA and protein analysis with excellent sensitivity. The detection limit for DNA quantitation in 384-well UV Microplates is 60 ng/ml and spectral analysis of DNA in 96-well UV Microplates can be performed with samples clearly smaller than 2 µg of DNA. For BSA quantitation in 96-well UV Microplates, assay detection limit is 7 µg/ml using results as such, but can be improved to 4 µg/ml by using pathlength correction function of the instrument. The stable and low background of both instrument and microplates enables to measure small sample concentrations with low volumes and so to save valuable sample material.