High-Throughput Enzyme Linked Immunosorbent Assay Performed by Molecule Immobilization on the Surface of Magnetic Particles

Reija-Riitta Harinen, Sini Suomalainen and Virpi Puro, Thermo Fisher Scientific, Sample Preparation & Analysis, Vantaa, Finland

This paper describes the detection of atrazine using enzyme linked immunosorbent assay (ELISA) by molecule immobilization on the surface of magnetic particles, and the separation of the particle bound complexes with the Thermo Scientific KingFisher Flex instrument, a magnetic particle processor. The ELISAs based on magnetic particle separation technique are faster to perform than the traditional ELISAs, which require more time-consuming separation steps of the unbound molecules.